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基于数据库筛选获得6种花青素与2型糖尿病共同靶点，构建花青素-共同靶点互作网络和蛋白质相互作用网络(PPI),并进行KEGG通路富集分析和GO功能注释分析，最后通过分子对接验证关键组分与核心靶点作用。共筛选出57个花青素与2型糖尿病的共同作用靶点。关键花青素组分为芍药色素、矢车菊素、锦葵色素和矮牵牛色素，核心靶点为AKT1、EGFR和ESR1。KEGG通路与GO功能富集分析表明，花青素可能通过基因表达负调控、信号转导、凋亡过程的负调控等生物学过程改善2型糖尿病。分子对接结果显示，4种花青素与3个靶点对接良好。其中，芍药色素与ESR1的结合构象最稳定，结合能为-7.40 kcal/mol。研究预测花青素通过调控多靶点、多通路机制改善2型糖尿病，为其相关分子研究提供了理论依据。
Abstract：Based on database screening, the intersecting targets of 6 types of anthocyanins and type 2 diabetes mellitus were obtained. An anthocyanin-common target interaction network and a protein-protein interaction(PPI) were constructed, and KEGG pathway enrichment analysis and GO functional annotation analysis were conducted. Finally, the key components and core targets were verified through molecular docking. A total of 57 common interaction targets of anthocyanins and type 2 diabetes were identified. The core anthocyanins were identified as peonidin, cyanidin, malvidin, and petunidin, while the key targets were determined to be AKT1, EGFR, and ESR1. KEGG pathway and GO functional enrichment analysis indicated that anthocyanins might improve type 2 diabetes mellitus through pathways such as the negative regulation of gene expression, signal transduction and negative regulation of apoptotic process. Molecular docking results showed that 4 types of anthocyanins had good binding activity with 3 targets. Among them, the binding conformation of peonidin with ESR1 was the most stable, with a binding energy of-7.4 kcal/mol. This study predicts that anthocyanins can improve type 2 diabetes through multi-target and multi-pathway mechanisms, providing a theoretical basis for related molecular research.
[ 1 ] 李玲玉，朱文卿，朱姗姗，等.基于网络药理学分析咖啡酰奎宁酸类化合物治疗Ⅱ型糖尿病的作用机制[J].食品工业科技，2021,42(14):16-24.
[ 2 ] TECK J.Diabetes-associated comorbidities[J].Primary Care:Clinics in Office Practice,2022,49(2):275-286.
[ 3 ] 焦宏伟.芍药色素对2型糖尿病合并非酒精性脂肪肝小鼠的保护作用[J].医药导报，2020,39(4):436-440.
[ 4 ] LEWIS A,WILLIAMS K,OROSZI T.Metformin—Pharmacokinetic and pharmacodynamics journey through the body[J].Pharmacology & Pharmacy,2024,15(12):466-477.
[ 5 ] 饶翀，崔丽梅，肖新华.二甲双胍胃肠道不良反应及其新型缓释制剂的临床应用[J].中华糖尿病杂志，2024,16(7):827-831.
[ 6 ] 郭芳瑜，张南海，邹晴如，等.鸢尾苷对晚期糖基化终末产物形成的抑制作用[J].食品工业科技，2024,45(24):98-106.
[ 7 ] KOWALCZYK T,MUSKA?A M,MERECZ-SADOWSKA A,et al.Anti-inflammatory and anticancer effects of anthocyanins in in vitro and in vivo studies[J].Antioxidants,2024,13(9):1143.
[ 8 ] MERECZ-SADOWSKA A,SITAREK P,KOWALCZYK T,et al.Food anthocyanins:Malvidin and its glycosides as promising antioxidant and anti-inflammatory agents with potential health benefits[J].Nutrients,2023,15(13):3016.
[ 9 ] YE X,CHEN W,HUANG X F,et al.Anti-diabetic effect of anthocyanin cyanidin-3-O-glucoside:data from insulin resistant hepatocyte and diabetic mouse[J].Nutrition & Diabetes,2024,14(1):7.
[10] REN L C C,TAN N X,OUYANG J,et al.Hypoglycaemic activity of the anthocyanin enriched fraction of Lycium ruthenicum Murr.fruits and its ingredient identification via UPLC-triple-TOF-MS/MS[J].Food Chemistry,2024,461:140837.
[11] 张亚珍，宋杰峰，吴煌福，等.miR-324-3p靶向AKT1调控乳腺癌细胞放射增敏性的机制研究 [J].中国临床研究，2020,33(7):879-883,889.
[12] SONG J J,LEE Y J.Dissociation of Akt1 from its negative regulator JIP1 is mediated through the ASK1-MEK-JNK signal transduction pathway during metabolic oxidative stress:a negative feedback loop[J].The Journal of Cell Biology,2005,170(1):61-72.
[13] CAO S R,PAN Y,TANG J Q,et al.EGFR-mediated activation of adipose tissue macrophages promotes obesity and insulin resistance[J].Nature Communications,2022,13(1):4684.
[14] GREGORIO K C R,LAURINDO C P,MACHADO U F.Estrogen and glycemic homeostasis:The fundamental role of nuclear estrogen receptors ESR1/ESR2 in glucose transporter GLUT4 regulation[J].Cells,2021,10(1):99.
[15] 李珊珊，郝义，张婷婷，等.基于主成分分析法分析不同采收期对李果实贮藏品质的影响[J].食品工业科技，2024,45(16):272-281.
[16] LEGIAWATI L.The role of oxidative stress,inflammation,and advanced glycation end product in skin manifestations of diabetes mellitus[J].Current Diabetes Reviews,2022,18(3):87-92.
[17] ANASTASIOU I A,TENTOLOURIS K N,LAMBADIARI V,et al.Effects of advanced glycation end products via oxidative stress on beta cells:Insights from in vitro and in vivo studies and update on emerging therapies[J].Mini Reviews in Medicinal Chemistry,2023,23(21):2041-2052.
[18] YUNG J H M,GIACCA A.Role of c-Jun N-terminal kinase (JNK) in obesity and type 2 diabetes[J].Cells,2020,9(3):706.
[19] 彭川，冉思燕，何苗，等.中药复方基于细胞信号通路防治2型糖尿病的研究进展[J].世界科学技术-中医药现代化，2024,26(6):1497-1504.
[20] 刘妍，常丽萍，高怀林.胰岛素抵抗的发病机制研究进展[J].世界中医药，2021,16(11):1671-1674.
[21] 黄好，贾虹，王晓霜，等.妊娠期糖尿病患者胎盘组织中miRNA-508-3p和HGF表达水平及其对滋养细胞胰岛素抵抗的影响[J].吉林大学学报(医学版),2021,47(1):187-195.
[22] ZHANG N,LIU X Y,ZHUANG L L,et al.Berberine decreases insulin resistance in a PCOS rats by improving GLUT4:Dual regulation of the PI3K/AKT and MAPK pathways[J].Regulatory Toxicology and Pharmacology,2020,110:104544.
[23] 刘莉，李卫忠，王师菡，等.中医药多靶点干预代谢综合征机制研究概述 [J].中国实验方剂学杂志，2021,27(3):214-221.
[24] ZHOU M,HOU Y L,WU J,et al.miR-93-5p promotes insulin resistance to regulate type 2 diabetes progression in HepG2 cells by targeting HGF[J].Molecular Medicine Reports,2021,23(5):1-12.
[25] 张永杰，吴致远，董玲玲，等.GSK-3β对缺血性脑卒中病理过程调控作用的研究进展[J].中国细胞生物学学报，2020,42(7):1263-1268.
[26] WANG B Y,LIU W Y,SUN F X.Nucleosome assembly protein 1-like 5 alleviates Alzheimer’s disease-like pathological characteristics in a cell model[J].Frontiers in Molecular Neuroscience,2022,15:1034766.
[27] DEMIR S,NAWROTH P P,HERZIG S,et al.Emerging targets in type 2 diabetes and diabetic complications[J].Advanced Science,2021,8(18):2100275.
[28] PAPADOPOULOS K I,PAPADOPOULOU A,AW T C.MicroRNA-155 mediates endogenous angiotensin II type 1 receptor regulation:implications for innovative type 2 diabetes mellitus management[J].World Journal of Diabetes,2023,14(9):1334-1340.
[29] MU J M,LIU Y,ZHANG F F,et al.Relationship between circular RNA and type 2 diabetes and its clinical application[J].Journal of China Pharmaceutical University,2020,51(3):374-378.
[30] 鄢月明，代瑛琦，邱书凯，等.基于网络药理学和分子对接探究菟丝子治疗白癜风的分子机制[J].湖北理工学院学报，2024,40(6):61-67.
[31] 杨祥军，申意伟，王丛悦，等.基于网络药理学和分子对接研究路路通治疗类风湿关节炎的分子机制[J].现代药物与临床，2024,39(12):3058-3064.
[32] 赵钜阳，方胥伟，顾丽雅，等.基于网络药理学及分子对接技术研究槐花的抗肥胖作用机制[J].食品工业科技，2024,45(24):42-49.
[33] 梁鑫富，董庆亮.光谱法结合分子对接技术研究柠檬苦素与牛血清白蛋白的相互作用[J].食品工业科技，2024,45(21):37-44.
[34] 唐高俊，胡杰，张宝芳.基于网络药理学和生物信息学探讨葛根芩连方治疗非酒精性脂肪性肝病的作用机制[J].中西医结合慢性病杂志，2024,1(1):26-39.
基本信息:
中图分类号:R587.1;TS201.4
引用信息:
[1]陈子萱,叶文倩,伍庆媛,等.基于网络药理学/分子对接联合法探究花青素改善2型糖尿病的作用机制[J].粮食与油脂,2025,38(10):133-140.
基金信息:
湖北省自然科学基金(2024AFB964); 湖北省教育厅科学研究计划青年项目(Q20231605); 湖北省自然科学基金联合基金项目(2024AFD079); 校级大学生创新创业训练计划项目(202510496006)
2025-10-10
2025-10-10